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MAHMOOD CHAMANKHAH
Nanobiotechnology Research Center, Avesina Research Institute, Tehran, Iran Tel: 98-21-2240-2011 Email: chamankh@duke.usask.ca
Education
Sep 94-Dec 98 Doctor of Philosophy (Ph.D. in Pharmaceutical Biotechnology) Department of Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, Canada Advisor: Dr. Wei Xiao Jun 92-Jun 94 Master of Science (M.Sc. in Medicinal Chemistry)College of Pharmacy and Nutrition, University of Saskatchewan Saskatoon, Canada Advisor: Professor Jonathan R. Dimmock
Jan 82-May 88 Doctor of Pharmacy (Pharm.D.) Faculty of Pharmacy, Mashhad University of Medical Sciences (MUMS) Mashhad, Khorasan, Iran
Job Experience
May 2004-Now Assistant Professor and Head Nanobiotechnology Research Center Avesina Research Center Tehran, Iran
July 2003-May 2004 Assistant Professor Department of Biotechnology Faculty of Sciences Tehran University Tehran, Iran
Jan 2003-July 2003 Assistant Professor Department of Immunology Shiraz University of Medical Sciences (SUMS) Shiraz, Iran
Mar 2000-July 2002 Professional Research Associate Saskatoon Research Center Agriculture and Agri-Food Canada Saskatoon, Canada
Mar 1999-Mar 2000 Postdoctoral Fellow Saskatoon Research Center Agriculture and Agri-Food Canada Saskatoon, Canada
Sep 94-Feb 99 Research Assistant Department of Microbiology and Immunology University of Saskatchewan, Saskatoon, Canada Advisor: Dr. Wei Xiao
Sept 96-Apr 97 Lecturer: Microbial Genetics College of Medicine, Health Sciences Building University of Saskatchewan Saskatoon, Canada
Sept 94-Apr 97 Teaching Assistant Pharmaceutical Analysis College of Pharmacy and Nutrition, University of Saskatchewan Saskatoon, Canada
May 89-May 92 Lecturer: Medicinal Chemistry and Pharmacology College of Pharmacy, Mashhad University of Medical Sciences (MUMS) Mashhad, Iran
Awards and Scholarships
Mar 99-Present NSERC Fellowship Award Sept 97-Dec 99 College of Medicine Graduate Teaching Fellowship, University of Saskatchewan, Saskatoon, Canada Sept 94-Sept 97 National Cancer Institute of Canada (NCI) Graduate Fellowship Jun 92- Jun 97 Scholarship, Ministry of Health and Medical Education, Tehran, Iran
Technical Competencies
2D Gel Electrophoresis of Proteins: Two Dimensional Gel Electrophoresis of Proteins, Peptide Mapping and Sequencing of Putative Protein Spots Using Mass Spectrometer Protein Expression: Protein Expression in a variety of systems such as bacterial, Baculovirus in sf9 and sf21 cells, Secreted proteins in the yeast Pichia pastoris Protein Purification: Purification of tagged proteins (His-tag or GST fusion) using Ni and GST columns, FPLC purification of proteins using ion-exchange, size-exclusion, and affinity columns Chromatography: FPLC purification of proteins using DEAE, SEC and Affinity columns Protein Interactions: Yeast two-hybrid filter and liquid assays, Domain mapping using two-hybrid assay, Co-immunoprecipitation Protein analysis: Protein extraction, SDS PAGE analysis, Western, Enzyme assays, Protease in-gel assay, ELISA, Posttranslational modifications (Glycosylation) Immunolocalization: Tissue and Cell preparations for Immunofluorescence and Immunogold Molecular Biology: Cloning and sublcloning, Restriction mapping, Sequencing, Southern and Northern blotting and hybridization, Colony hybridization and screening,, Screening of cDNA and genomic libraries in Bacterial and phage vectors 5’ and 3’ RACE, analysis of prokaryotic and eukaryotic gene expression, Damage- and stressed-induced expression Genomic and cDNA template PCR amplification, colony PCR screening, Degenerate PCR cloning Transformation and Transfection. Phage Display: Phage display technique and its application in screening a library of ScFv antibody fragments with high affinity toward a target protein or peptide, Humanization of Mouse Recombinant Antibody Radioisotopes: Radioisotope techniques, labeling of nucleic acids and proteins Spectrophotometry: UV/Vis, Fluorometry Computer (general): IBM-PC and Apple Mac applications, Endnote, MS Office, Groupwise, DOS/Windows 95, 98, NT and Unix/Mac operating systems, Home page design Computer (scientific): GCG, PubMed and Genbank database search, DNA STAR, Sigma Plot and Cricket Graph, ISIS Base/Draw, Scanning & densitometric analysis Industry experience: Biosafety, Chemical and Radiation safety, GLP Approved Research Projects
2) Production of recombinant human single chain monoclonal antibodies (scFv) against hepatitis B surface antigen for therapeutic applications 3) Production of Recombinant Human Antibodies for the Immunotherapy of Cancer and Chronic Diseases: Human Antibody Against Hepatitis B Surface Antigen 4) Molecular Cloning, Expression and Purification of Recombinant Inhibin a Subunit 5) Conjugation of Nonomagnetic Particles to Monoclonal Antibodies and Peptides: Optimization and Application in Cancer Diagnosis and Treatment 6) Application of Nanoparticle Quantum Dots in the Early Diagnosis of Two Most Common Ovarian and Breast Cancers in Iran 7) Synthesis, Organic Functionalization and Biomolecule Conjugation of Semiconductor Nanoparticle Quantum Dots: Application in Diagnosis of Prostate Cancer Publications
1. Chamankhah M, Hegedus D, Characterization of integral proteins of Mamestra configurata peritrophic matrix using a proteomics approach (In preparation for Mol. Cell. Proteomics). 2. Chamankhah M, Hegedus D, Expression, purification and biochemical characterization of novel Serpins from peritrophic matrix of Mamestra configurata. (In preparation for J. Biol. Chem). 3. Shi X, Chamankhah M, Visal-Shah S, Hemmingsen SM, Erlandson M, Braun L, Alting-Mees M, Khachatourians GG, O'grady M, Hegedus DD. (2004) Modeling the structure of the Type I peritrophic matrix: characterization of a Mamestra configurata intestinal mucin and a novel peritrophin containing 19 chitin binding domains. Insect Biochem. Mol. Biol. 34:1101-15.
4.
Dimmock JR,
Chamankhah M, Das U, Zello GA, Quail JW, Yang J, Nienaber KH, Sharma
RK, Selvakumar P, Balzarini J, De Clercq E, Stables JP. (2004)
Cytotoxic and topographical properties of
6-arylidene-2-dimethylaminomethylcyclohexanone hydrochlorides and related
compounds.
5.
Shi X, Karkut T,
Alting-Meese M, Chamankhah M, Hemmingsen SM, Hegedus DD (2003)
Enhancing Escherichia coli electrotransformation competency by
invoking physiological adaptations to stress and modifying membrane
integrity. 6. Shi X, Karkut T, Chamankhah M, Alting-Meese M, Hemmingsen SM, Hegedus DD (2003 (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Protein Expr Purif. 28:321-30. 7. Chamankhah M, Braun L, Visal S, O’Grady M, Baldwin D, Shi X, Alting-Meese M, Hemmingsen SM, Hegedus DD (2003) Mamestra configurata Serpin-1 homologues: cloning, localization and developmental regulation. Insect Biochem. Mol. Biol., 33:355-369. 8. Shi X, Chamankhah M, Karkut T, Alting-Meese M, Hemmingsen SM, Hegedus DD (2003) Optimal conditions for the expression of a single-chain antibody (scFv) gene in Pichia pastoris. Prot. Exp. Purif. 28:321-330. 9. Shi X, Karkut T, Chamankhah M, Alting-Mees M, Hemmingsen SM, Hegedus DD (2002) 5'-RACEing across a bridging oligonucleotide. Biotechniques 32(3), 480-482. 10. Chamankhah M, Visal S, O’Grady M, Braun L, Hemmingsen SM, Hegedus DD, A proteomics approach to the analysis of peritrophic matrix proteins of Mamestra configurata. (In preparation for Insect Mol Biol) 11. Chamankhah M, Fontanie T, Xiao W (2000) The Saccharomyces cerevisiae mre11(ts) allele confers a separation of DNA repair and telomere maintenance functions. Genetics. 155:569-576 12. Chamankhah M, Xiao W (1999) Formation of the yeast Mre11-Rad50-Xrs2 complex is correlated with DNA repair and telomere maintenance. Nucleic Acids Research 27:2072-2079. 13. Chamankhah M, Xiao W (1998) Isolation of hMRE11B: failure to complement yeast mre11 defects due to species-specific protein interaction, Gene 225:107-116. 14. Chamankhah M, Xiao W (1998) Molecular cloning and genetic characterization of the Saccharomyces cerevisiae NGS1/MRE11 gene, Current Genetics 34:368-374. 15. Dimmock JR, Kandepu NM, Hetherington M, Quail JW, Pugazhenthi U, Sudom AM, Chamankhah M, Rose P, Pass E, Allen TM, Halleran S, Szydlowski J, Mutus B, Tannous M, Manavathu EK, Myers TG, De Clercq E, Balzarini J (1998) Cytotoxic activities of Mannich bases of chalcones and related compounds. J. Med. Chem. 41, 1014-26. 16. Dimmock JR, Chamankhah M, Seniuk A, Allen TM, Kao GY, Halleran S (1995) Synthesis and cytotoxic evaluation of some Mannich bases of alicyclic ketones. Pharmazie 50:668-71. 17. Dimmock JR, Chamankhah M, Allen TM, Halleran S (1995) Mannich bases of 2-arylmethylenecyclohexanone with cytotoxic activity. Pharmazie 3:221-2.
Selected Scientific Presentations, Abstracts
1. Chamankhah, M., Xiao, W. Telomere Function of Mre11. Conference on Telomeres and Telomerases. August 24-29 2001, Vancouver, BC, Canada. 2. Chamankhah, M., Xiao, W. Molecular Cloning of the Yeast and Human NGS1 and Characterization of their Interacting Domains. Abstract 3004. 17th International Congress of Biochemistry and Molecular Biology. August 24-29 1997, San Francisco, California, USA. 3. Chamankhah, M., Xiao, W. Characterization of the Yeast and Human NGS1, an Allele of MRE11. Abstract 188B. Yeast Genetics and Molecular Biology Meeting. August 6-11 1996, University of Wisconsin, Madison, WI, USA. 3. Chamankhah, M., Rahimizadeh, M., Heravi, M. A New Method for Synthesis of Dantrolene and its Derivatives. Abstract 21. Seminars in Chemistry and Chemical Engineering, May 1986. Isfahan University, Isfahan, Iran.
Invention Disclosure
Chamankhah, M., Visal, S., Hemmingsen, S., Braun, L., Khachatourians, G., Hegedus, D. Novel members of Serpin family in the peritrophic matrix of Mamestra configurata. In preparation.
Contribution to the Training of Qualified Personnel
Graduate Students 1. Mr. Bryan Sarauer, M.Sc. candidate, Department of Applied Microbiology and Food Science, University of Saskatchewan, Canada 2. Mr. Landon Pastushok, Ph.D. candidate, Department of Microbiology and Immunology, University of Saskatchewan, Canada
Research Technicians 1. Ms. Treena Fontanie, Department of Microbiology and Immunology, University of Saskatchewan, Canada 2. Mr. Doug Baldwin, Saskatoon Research Center, Agriculture and Agri-Food Canada, Saskatoon, Canada 3. Mrs. Mina Faghih, Technician, Department of Applied Microbiology and Food Science, University of Saskatchewan, Canada
References
Dr. Dwayne Hegedus Group Leader, Molecular Plant Protection, Molecular Genetics Section, Agriculture and Agri-Food Canada Saskatoon Research Center, Saskatoon, SK, Canada Phone: (306) 956-7667, FAX: (306) 956-7247, Email: hegedusd@gc.agr.ca Adjunct Professor, Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, Canada
Dr. Wei Xiao Associate Professor, Department of Microbiology and Immunology, College of Medicine, University of Saskatchewan, Saskatoon, SK, Canada Phone: (306) 966-4308, FAX: (306) 966-4311, Email: xiaow@sask.usask.ca
Dr. Sean Hemmingsen Professor, Plant Biotechnology Institute (PBI), National Research Council (NRC) of Canada, Saskatoon, SK, Canada Phone: (306) 975-5242, FAX: (306) 975-2222, Email: hemmings@cbrpbi.pbi.nrc.ca
Dr. Gerry Rank Professor, Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada Phone: (306) 966-4415, FAX: (306) 966-4461, Email: rank@skyway.usask.ca
Dr. Jonathan. R. Dimmock Professor, Division of Pharmaceutical Sciences, College of Pharmacy and Nutrition, University of Saskatchewan, Saskatoon, SK, Canada Phone: (306) 966-6331, FAX: (306) 966-6377, Email: dimmock@skyway.usask.ca
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