1997 - 1998           Centro de Ingenieria Genetica y Biotenologia                 Havana, Cuba

PostDoc, Protein Characterization

1990 - 1994        Faculty of Medicine, Tehran Medical University            Tehran, Iran

Ph.D., Immunology                  

1984 - 1990        Faculty of Pharmacy, Tehran Medical University           Tehran, Iran

Pharm.D.          

1998 - Date           Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran

Assistant Professor

Establish the protein chemistry unit. The settled techniques are:

1. Amino acid analysis with pre- and post- column derivatization.

2. Reverse Phase HPLC for proteins and peptides.

3. Liquid chromatography for protein purification.

3. 2-Dimensional Electrophoresis by IPG- SDS PAGE.

4. Circular Dichroism spectropolarimeter for evaluation the second structure of proteins.

1997-1998          Centro de Ingenieria Genetica y Biotenologia, Havana, Cuba

Postdoctoral Training

Characterization of sea anemona protease inhibitor isomers.

Amino acid composition analysis.

Automatic protein and peptide N-terminal sequencing.

Peptide sequencing by Mass Spectrometry.

Protein identification by database searching.

Recombinant protein stability analysis.

Characterization and establishment of biological reference material.

1994 – 1997        Immunology Department, Artesh Medical University, Tehran, Iran

Faculty Member

Design of immunology laboratory for medical immunology course.

Design of cell culture laboratory for human lymphocyte cell culture.

Study of the peripheral lymocytes apoptosis in patients with acute lymphoproliferative lymphoma by flowcytometric DNA analysis.

1990 -1994   Department of Immunology,Tehran Medical University,Tehran, Iran

Ph.D. Candidate

Study of the in vivo and in vitro effects of Lithium Chloride on the human polymorphonuclears (PMNs) by chemoluminometery measurement of the oxidative burst and evaluation of the PMNs’ killing index.

1.    Protein mapping of Promastigote and Amastigote Forms of Leishmania Major by Two Dimensional Electrophoresis.

2.    Two Dimensional Electrophoresis Protein Mapping of BHK Cells Before and After Infection by Rabies Virus.

3.    Determining new auto-antibodies in sera of patients with autoimmune hepatitis type I using two-dimensional electrophoresis protein profile of Hep-2 cells. (In collaboration with research center of gastroenterology and liver transplantation)

4.    Evaluation a standard analytical assay method for a synthetic analogue of gonadotropin-releasing hormone.

5.    Identifying the Monooxygenase Activity in the Bacteria sample “RIPI-F”: Purification and characterization of the enzyme. (In collaboration with Research Institute of Petroleum Industry)

1. Hediyeh Rasam ,Sarvnaz Hashemi, Alireza Gholami, Maryam Zakeri and Behrouz Vaziri (2005)A prefractionation strategy for protein profiling of leishmania major promastigotes by two-dimensional electrophoresis. Cancer Genomics and proteomics 2. abstracts of firstHellenic proteomics Congress, Greece.

2. Alireza Gholami, Sarvnaz Hashemi, Ahmad Adeli, and Behrouz Vaziri. Comparison of two different sample preparation methods for establishing carrier ampholyte based two-dimensional electrophoresis pattern of the Leishmania major promastigotes. In proceeding of the third International Congress of Biotechnology, Mashad, Iran. Sep. 2003.

3. Maryam Zakeir, and Behrouz Vaziri. Optimization of Acid Hydrolysis and Comparison of Two Pre-column Derivatization methods for Amino Acid Analysis of a Gonadotropin Releasing Hormone Analogue. In proceeding of the third International Congress of Biotechnology, Mashad, Iran. Sep. 2003.

4. Afshin Peirovi, Maryam Zakeri, and Behrouz Vaziri. A reverse phase high performance liquid chromatography method for analytical assay of a synthetic analogue of gonadotropin-releasing hormone. In proceeding of the third International Congress of Biotechnology, Mashad, Iran. Sep. 2003.

5. Vaziri, B. Post-genome era: emergence of proteomics industry for high throughput structural and functional characterization of proteins, Second International Congress of Biotechnology, Karaj, Iran. Nov. 2001.

6. Zakeri M and Vaziri B. Evaluation of purity and amino acid composition of a synthetic peptide analogue of Leutizing Hormone Releasing Hormone.  Second International Congress of biotechnology, Karaj, Iran. Nov. 2001.

7. Vaziri B and Zakeri M. 2001. Manual of current amino acid analysis methods for evaluation of proteins.

8. Vaziri B. Application of mass spectrometry in characterization of peptides and proteins.( manuscripts in preparation)

9. Vaziri B . Practical Guide for Reverse Phase-HPLC Applications in Proteins and Peptides.( manuscripts in preparation)

10.                  Vaziri B and Masoud M. 1996. In vivo and in vitro effects of Lithium Chloride on human neutrophils. Third International Congress of Immunology and Alergy, Shiraz, Iran